Partial Hepatic Ischemia-Reperfusion Model
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Corresponding Organization :
Other organizations : University of Pittsburgh, North Shore University Hospital, New York University
Protocol cited in 13 other protocols
Variable analysis
- Duration of hepatic ischemia (60 minutes)
- Visualized pale blanching of the ischemic lobes (during ischemia)
- Immediate color change on reperfusion (after clamp removal)
- Mortality (mice excluded from further analysis if no ischemic color changes or lack of response to reperfusion)
- Anesthesia (sodium pentobarbital and methoxyflurane)
- Temperature (maintained at 37°C by warming pad and heat lamp)
- Surgical procedure (midline laparotomy, dissection of liver hilum, use of microvascular clamp)
- Ischemic model (segmental, 70% of the liver)
- Visualization of pale blanching of the ischemic lobes during the ischemic interval
- Immediate color change on reperfusion after clamp removal
- Mice with no ischemic color changes or lack of response to reperfusion (excluded from further analysis)
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