Longitudinal Infant Macaque Blood Study

Animals were immobilized with (10 mg/kg) ketamine-HCl intramuscularly (IM) prior to all sample collections. Complete blood counts (CBC) were performed at each blood collection. Infant EDTA blood samples were collected longitudinally starting at birth and subsequently every month for one year. Plasma was removed from whole blood by centrifugation and stored at −80°C for antibody measurement. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood via density centrifugation using Lymphocyte Separation Medium (LSM) (MP Biomedicals, Solon, OH) or Accu-Paque Lymphocyte separation media (Accurate Chemical & Scientific Corp., Westbury, NY) as described [51 (link)-53 (link)]. Due to the low blood volume that could be collected from infant macaques, and therefore relatively low cell counts, infant PBMC were used immediately for functional analyses. PBMC from adolescent and adult macaques were cryopreserved for functional assays and batch-analysis.

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Oxford K.L., dela Pena-Ponce M.G., Jensen K., Eberhardt M.K., Spinner A., Van Rompay K.K., Rigdon J., Mollan K.R., Krishnan V., Hudgens M.G., Barry P.A, & De Paris K. (2015). The interplay between immune maturation, age, chronic viral infection and environment. Immunity & Ageing : I & A, 12, 3.

Publication 2015

Lymphocyte Separation Medium (LSM)

Adolescent Adult Animals Antibody Assays Birth Blood Blood volume Centrifugation Edta Infant Ketamine hcl Lymphocyte Macaques Peripheral blood mononuclear cells Plasma Sample collections Solon

Corresponding Organization :

Other organizations : University of California, Davis, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

Ketamine-HCl (10 mg/kg) administered intramuscularly (IM)

dependent variables

Complete blood counts (CBC)
Antibody measurements in plasma
Functional analyses of peripheral blood mononuclear cells (PBMC)

control variables

Blood collection time points: At birth and every month for one year
Blood sample processing: Plasma separation, PBMC isolation, and storage conditions

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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