Cell cycle analysis and apoptosis rates following VIIb treatment were investigated using a Propidium Iodide Flow Cytometry Kit (ab139418; Abcam, Cambridge, UK) [60 (link)] and Annexin V-FITC apoptosis kit (Catalog: K101-25; BioVision Research Products, San Francisco, CA, USA) [61 (link)], respectively, following the manufacturer’s instructions as stated by a previous study [17 (link)].
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