Screening Surface Markers of hMSCs

To screen the surface markers of hMSCs, 242 antibodies (Figure S1) were lyophilized in 96-well plates (BD Lyoplates; BD Biosciences) at 0.5 µg/well and incubated with 500,000 MSCs per well. After 20 min of reconstitution on ice, the washed cells were stained with an Alexa Fluor^® 647-conjugated goat anti-mouse IgG secondary antibody (Molecular Probes, Eugene, OR, USA). Flow cytometry was performed to confirm the surface marker expression on a FACSCalibur instrument (BD Biosciences). Flow cytometry data were analyzed using Excel 2013 (Microsoft, Redmond, WA, USA) to generate heat maps [28 (link)].