The amounts of IFN-γ and IL-17A in the cell culture supernatants were determined based on a previously described method30 (link). Spleens were aseptically removed, and cells were suspended at a concentration of 2 × 106 cells/mL in complete media (RPMI 1640 with 10% FBS). The cells were stimulated with or without 10 μg/mL of IsdB, ClfA33-213, or IC protein at 37 °C for 5 days. The supernatants were collected, and the amounts of IFN-γ and IL-17A were determined by ELISA using mouse IFN-γ ELISA and IL-17A ELISA kits (Biolegend), respectively.
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