Serum Biochemistry Analysis for Diabetes Research

Serum samples from the chosen patients were allowed to defrost prior to biochemical analysis. HbA1c was analyzed directly using ion-exchange high-performance liquid chromatography (HPLC) with commercially available reagents (D-10 Hemoglobin A1c Program #12000949) in a fully automated system (D-10 Hemoglobin Testing System #12010405, Bio-Rad, Hercules, CA, USA). Fasting serum insulin levels were analyzed using a Luminex multiplex system (Luminex Corp, Austin, TX, USA) and a commercially available kit, catalog no. HINS-MAG. The intra-assay % CV was <10 and the inter-assay % CV was <15, according to the manufacturer’s protocol. Serum glucose, triglyceride, cholesterol and high-density lipoprotein (HDL) levels were analyzed regularly using commercially purchased kits (catalog nos. 981812, 981379, 981823 and 981301, respectively) and an automated biochemical analyzer (Konelab 20 Thermo Fischer, Espoo, Finland), as we described previously [23 (link)].

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Alfadul H., Sabico S., Alnaami A.M., Amer O.E., Hussain S.D., Wani K., Clerici M, & Al-Daghri N.M. (2023). Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins. International Journal of Molecular Sciences, 24(18), 13837.

Publication 2023

D-10 Hemoglobin A1c Program #12000949 D-10 Hemoglobin Testing System Konelab 20

Assay Austin Cholesterol Glucose Hemoglobin Hemoglobin a1c High density lipoprotein High performance liquid chromatography Insulin Ion exchange Patients Serum Triglyceride

Corresponding Organization : King Saud University

Other organizations : University of Milan

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

HbA1c
Fasting serum insulin levels
Serum glucose
Serum triglyceride
Serum cholesterol
Serum high-density lipoprotein (HDL) levels

control variables

Serum samples from chosen patients were allowed to defrost prior to biochemical analysis
Commercially available reagents and kits were used for analyses
Analyses were performed in fully automated systems and an automated biochemical analyzer
Intra-assay % CV was <10 and inter-assay % CV was <15 for fasting serum insulin levels, according to the manufacturer's protocol

controls

None explicitly mentioned

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