Sections were cut from paraffin blocks of the muscle (TBD) tissue using a rotary microtome (Leica Microsystems). After sectioning, each slide was coded as a number for blind evaluation. Each number was generated using the RAND function of the Excel software, sorted in ascending order, and assigned to the slides. The tissue slides were used for staining and evaluated by an experimenter.
For HE staining, sections were cut from frozen blocks of muscle (tibialis anterior) tissue and stained with Lillie-Mayer’s haematoxylin (Muto Pure Chemicals Co., Ltd., Japan) and eosin solution (FUJIFILM Wako Pure Chemical Corporation). The inflammation score was calculated according to the criteria of Tinsley9 (link), as shown below:
For HE staining, sections were cut from frozen blocks of muscle (tibialis anterior) tissue and stained with Lillie-Mayer’s haematoxylin (Muto Pure Chemicals Co., Ltd., Japan) and eosin solution (FUJIFILM Wako Pure Chemical Corporation). The inflammation score was calculated according to the criteria of Tinsley9 (link), as shown below:
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