Serum Ang-2 Quantification by ELISA

The patients’ blood was collected prior to therapy in serum-gel monovettes (Sarstedt, Germany) and was prepared as previously described (Ding et al. 2022 (link)). All serum samples were stored at − 80 °C and thawed on ice right before measurement. Serum Ang-2 was measured with the Human Angiopoietin-2 Quantikine ELISA set (R&D Systems, Minneapolis, Minnesota, USA, Catalog No. DANG-20). Serum samples were diluted 1:5. The procedure was performed according to protocol provided by the manufacturers.

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Volk A., Legler K., Hamester F., Kuerti S., Eylmann K., Rossberg M., Schmalfeldt B, & Oliveira-Ferrer L. (2023). Ang-2 is a potential molecular marker for lymphatic metastasis and better response to bevacizumab therapy in ovarian cancer. Journal of Cancer Research and Clinical Oncology, 149(17), 15957-15967.

Publication 2023

serum-gel monovettes

Blood Elisa Human angiopoietin 2 Patients Serum Therapy

Corresponding Organization : Universität Hamburg

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum Angiopoietin-2 (Ang-2) levels

control variables

Serum samples were collected prior to therapy
Serum samples were stored at -80°C
Serum samples were thawed on ice before measurement
Serum samples were diluted 1:5

controls

None explicitly mentioned

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