Stabilized HIV-1 gp140 Env Trimer Production
Corresponding Organization : Frederick National Laboratory for Cancer Research
Protocol cited in 1 other protocol
Variable analysis
- Envs were expressed as chimeric SOSIPs
- The gp120 of CH0848 was grafted onto the BG505 gp41
- The trimers were stabilized with E64K and A316W mutations
- Protein expression and purification
- Proteins were expressed in Freestyle293 cells (Invitrogen) using transient cotransfection of Env and furin DNA
- Cell-free culture media was subjected to PGT151 antibody affinity and superdex200 size exclusion chromatography
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