ICRF-159 and Thymidine Treatment of HeLa Cells

HeLa cells were cultured in DMEM supplemented with 10% FCS as previously described [16 (link)].
ICRF-159 (Razoxane) was provided by Sigma Aldrich (R8657) and was added to the cell culture medium at a final concentration of 1 or 10 µM following the protocol described in figures 1a, 2c, 3a, 4a and electronic supplementary material, figure S2A. Thymidine was provided by Sigma Aldrich (T9250) and was added to the cell culture medium at a final concentration of 2 mM.
All cells were routinely checked for mycoplasma infection.

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Gemble S., Buhagiar-Labarchède G., Onclercq-Delic R., Fontaine G., Lambert S, & Amor-Guéret M. (2020). Topoisomerase IIα prevents ultrafine anaphase bridges by two mechanisms. Open Biology, 10(5), 190259.

Publication 2020

ICRF-159 Thymidine

Cell culture Cells Culture medium Hela cells Icrf 159 Mycoplasma infection Razoxane Thymidine

Corresponding Organization : Université Paris Sciences et Lettres

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Variable analysis

independent variables

ICRF-159 (Razoxane) at a final concentration of 1 or 10 µM

dependent variables

Cell responses to ICRF-159 treatment

control variables

HeLa cells cultured in DMEM supplemented with 10% FCS
Thymidine added to the cell culture medium at a final concentration of 2 mM

controls

All cells were routinely checked for mycoplasma infection to ensure a negative control

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