Conidia Generation and Antifungal Assays for Penicillium Species

For generation of conidia, fungi were cultured on Potato Dextrose Agar (PDA; Difco-BD Diagnostics, Sparks, MD, USA) plates for 5 (P. chrysogenum strains)–7 (P. digitatum, P. italicum and P. expansum strains) days at 25 °C. For antifungal assays, P. digitatum CECT 20796 (PHI26) [32 (link)], P. italicum CECT 20909 (PHI1) and P. expansum CECT 20906 (CMP-1) [33 (link)] strains were used. Representative images of fungal growth on PDA plates are shown in Figure 1a.

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Gandía M., Kakar A., Giner-Llorca M., Holzknecht J., Martínez-Culebras P., Galgóczy L., Marx F., Marcos J.F, & Manzanares P. (2021). Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay. Journal of Fungi, 7(6), 449.

Publication 2021

Potato Dextrose Agar (PDA;

Agar Antifungal Assays Conidia Dextrose Diagnostics Fungi Potato Strains

Corresponding Organization : Consejo Superior de Investigaciones Científicas

Other organizations : Universitat de València, Innsbruck Medical University, Universität Innsbruck, HUN-REN Szegedi Biológiai Kutatóközpont, Institute of Plant Biology, University of Szeged

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Variable analysis

independent variables

Fungal species/strains: Penicillium chrysogenum, Penicillium digitatum, Penicillium italicum, Penicillium expansum
Culturing conditions: Potato Dextrose Agar (PDA) plates, 5-7 days at 25 °C

dependent variables

Generation of conidia (spores)

control variables

Potato Dextrose Agar (PDA) medium composition
Incubation temperature (25 °C)

controls

Positive control: None mentioned
Negative control: None mentioned

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