Bladder Cancer Mouse Model Development

KRT5-Cre^ERT2 and Tgfbr2^flox/flox mice were obtained from Jackson Laboratory, Balb/c male mice were provided by Shanghai Laboratory Animals Center (SLAC). BBN (Santa Cruz) was dissolved in drinking water at a concentration of 0.05% (w/v) and provided to transgenic or wild type male mice for 30 weeks as previous described38 (link). Tamoxifen (T5648) were purchased from Sigma, BBN(sc-486264) and LY 364947 (sc-203122) were obtained from Santa Cruz. All animal procedures were performed under a protocol approved by the Laboratory Animal Center of Anhui Medical University and in accordance with National Institutes of Health guide for the care and use of Laboratory animals (NIH Publications No. 8023, revised 1978).