Example 6
Optimization of Morphogen Exposure
The optimal duration of caudalization and ventralization may vary depending on the parent cell line used, culture conditions, and quality of reagents. For cells with ESC origin both caudalization and ventralization are typically 1 day faster, for hiPSC derived from adult cells, the time can depend on the origin of the somatic cells. Several different types of cells have been used to produce iPSCs, including fibroblasts, neural progenitor cells, keratinocytes, melanocytes, CD34+ cells, hepatocytes, cord blood cells and adipose stem cells. In hiPSC derived from CD34+ cells caudalization and ventralization may be slower for up to 2 days. hiPSC derived from fibroblasts typically follow the time line as explained in the
