Neonatal Pups Carboxyhemoglobin Assessment

To analyze the carboxyhemoglobin concentration, the 7-day-old neonatal pups were exposed to two inhaled doses of CO at 200 and 250 ppm. These chosen doses were based on animal models of lung injury and our laboratory's preliminary results, which show neuroprotection for stroke at these doses (16 (link), 18 (link), 19 (link)). The pups were placed in the hypoxic chamber. Thermoregulation of the pups was obtained using a gel pad (Deltaphase Isothermal Pad, Braintree Scientific Inc., Braintree, MA) during CO exposure. The pups were exposed to the CO for 1 h. A gas analyzer attached to the outflow will strictly monitor the CO levels in the chamber. Following CO exposure, the pups were sacrificed at 0, 10, 20, 60, 120, 180, and 240 min after completion of CO and blood was collected for carboxyhemoglobin analysis.

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Douglas-Escobar M., Mendes M., Rossignol C., Bliznyuk N., Faraji A., Ahmad A.S., Doré S, & Weiss M.D. (2018). A Pilot Study of Inhaled CO Therapy in Neonatal Hypoxia-Ischemia: Carboxyhemoglobin Concentrations and Brain Volumes. Frontiers in Pediatrics, 6, 120.

Publication 2018

Deltaphase Isothermal Pad

Animal models Blood Carboxyhemoglobin Hypoxic Lung injury Neonatal Neuroprotection Stroke Thermoregulation

Corresponding Organization : University of Florida

Other organizations : University of California, San Francisco

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Variable analysis

independent variables

Inhaled doses of CO at 200 and 250 ppm

dependent variables

Carboxyhemoglobin concentration

control variables

Thermoregulation of the pups using a gel pad during CO exposure
Monitoring of CO levels in the chamber using a gas analyzer

controls

No positive or negative controls were explicitly mentioned in the provided information.

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