In total, for 122 genes, we sequenced all exons including 20 flanking intronic base pairs. In addition, for the genes with a known cis-eQTL effect[15 (link)], we included 1000 base pairs upstream of the transcription start site in the sequencing design to enable us to identify regulatory variants in the promoter sequence of those genes.
Pooled targeted enrichment of DNA from 790 Dutch UC patients (12 individuals per pool) was performed using a custom-made kit (Agilent HaloPlex). The HaloPlex kit was designed with Agilent’s Sure Design, resulting in coverage of 99.9% of the target sequence (S1 File ).
Pooled targeted enrichment of DNA from 790 Dutch UC patients (12 individuals per pool) was performed using a custom-made kit (Agilent HaloPlex). The HaloPlex kit was designed with Agilent’s Sure Design, resulting in coverage of 99.9% of the target sequence (
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