Tumor cells were grown, harvested, and resuspended at 1 ×
104 cells/ml in complete RPMI 1640 medium. A total of 0.05 ml
of the cell suspension was added to flat bottomed 96-well plates, followed
by 0.05 ml of three-fold serial dilutions of POM esters. After incubation at
37°C with 5% CO2 for 4 days, 0.1 ml of
CellTiter-Glo reagent (Promega, Madison, WI, USA) was added and the
luminescence resulting from ATP was measured using an ARVO luminometer
(PerkinElmer, Foster City, CA, USA). All experiments were performed in
triplicate. The concentrations required for 50% tumor cell growth
inhibition (IC50 values) are shown. The sources for the cell
lines are detailed in Idrees et al.[30 (link)]
104 cells/ml in complete RPMI 1640 medium. A total of 0.05 ml
of the cell suspension was added to flat bottomed 96-well plates, followed
by 0.05 ml of three-fold serial dilutions of POM esters. After incubation at
37°C with 5% CO2 for 4 days, 0.1 ml of
CellTiter-Glo reagent (Promega, Madison, WI, USA) was added and the
luminescence resulting from ATP was measured using an ARVO luminometer
(PerkinElmer, Foster City, CA, USA). All experiments were performed in
triplicate. The concentrations required for 50% tumor cell growth
inhibition (IC50 values) are shown. The sources for the cell
lines are detailed in Idrees et al.[30 (link)]
