Protocol detail

Quantifying Desmoplastic Reaction in OSCC

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As described in a previous study, the desmoplastic reaction status was evaluated according to the ratio of the area of the tumour-associated stroma to that of the whole tumour tissue [32 (link)]. Histologic images were obtained using the light microscope (Olympus BX53®, Tokyo, Japan) with an attached digital camera (DP27®, Japan) and Olympus cellSens™ Entry software. The subjected areas were measured on haematoxylin and eosin-stained OSCC tumour tissue sections with ImageJ software. The desmoplasia status was further divided into two groups according to the calculated ratio: high (ratio ≥ 1) and low desmoplastic (ratio < 1) reaction.

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An Y.Z., Cho E., Ling J, & Zhang X. (2020). The Axin2-snail axis promotes bone invasion by activating cancer-associated fibroblasts in oral squamous cell carcinoma. BMC Cancer, 20, 987.

Publication 2020

cellSens™ Entry software

Camera Desmoplastic Eosin Haematoxylin Light microscope Stained tissue Tissue Tumour

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Variable analysis

independent variables

Desmoplastic reaction status

dependent variables

Ratio of the area of the tumour-associated stroma to that of the whole tumour tissue

control variables

Haematoxylin and eosin-stained OSCC tumour tissue sections
Light microscope (Olympus BX53®, Tokyo, Japan) with an attached digital camera (DP27®, Japan) and Olympus cellSens™ Entry software
ImageJ software for measuring the subjected areas

controls

No positive or negative controls were explicitly mentioned in the provided information.

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