Membrane Potential Analysis in Bacteria

For analyzing alterations in membrane potential, control and AA-treated bacteria were resuspended in 1 ml of PBS containing 30 μM of the potentiometric dye DiOC2(3) (BacLight Membrane Potential Kit, Molecular Probes, Life Technologies, Eugene, OR, USA) (Wolfson et al., 2023 (link)). The fluorescence intensities were measured in a Fortezza flow cytometer using the excitation/emission of 488 nm/530 nm for green fluorescence and 488 nm/620 nm for red fluorescence. A relative increase in red fluorescence in comparison to green fluorescence is a sign for membrane hyperpolarization.

Free full text: Click here

Chamlagain M., Hu J., Sionov R.V, & Steinberg D. (2024). Anti-bacterial and anti-biofilm activities of arachidonic acid against the cariogenic bacterium Streptococcus mutans. Frontiers in Microbiology, 15, 1333274.

Publication 2024

BacLight Membrane Potential Kit

Corresponding Organization : Hebrew University of Jerusalem

Other organizations : Hong Kong Baptist University, Nanyang Technological University, ShanghaiTech University

Top 5 similar protocols

Variable analysis

independent variables

Treatment with AA (arachidonic acid)

dependent variables

Membrane potential (measured by fluorescence intensity of the potentiometric dye DiOC2(3))

control variables

Bacteria were resuspended in PBS buffer
Concentration of the potentiometric dye DiOC2(3) was 30 μM
Fluorescence intensities were measured using a Fortezza flow cytometer with excitation/emission of 488 nm/530 nm for green fluorescence and 488 nm/620 nm for red fluorescence

controls

Control bacteria (not treated with AA)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!