SARS-CoV-2 Variant Neutralization Titers

SARS-CoV-2 neutralization titers, expressed as the ID₅₀, were assessed using pseudotyped lentiviruses presenting SARS-CoV-2 spike mutations for the D614G (Wuhan-1 containing a single D614G spike mutation), B1.617.2 (Delta), B.1.351 (Beta) and B.1.1.529 (Omicron BA.1) variants, as described previously^{22 (link),41 (link)}. A random subset of samples (25 per selected vaccine arm, distributed equally between age strata and sites) was analyzed for neutralization titers to the Omicron BA.4/BA.5, BA.2.12.1, BA.2.75.2, BA.2.75, BA.4.6, BF.7 and BQ.1.1 subvariants in a separate laboratory^{42 (link)}. Electrochemiluminescence immunoassays were used for the detection of anti-N (Elecsys Anti-SARS-CoV-2 N; Roche) at baseline^{41 (link)}.

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Branche A.R., Rouphael N.G., Diemert D.J., Falsey A.R., Losada C., Baden L.R., Frey S.E., Whitaker J.A., Little S.J., Anderson E.J., Walter E.B., Novak R.M., Rupp R., Jackson L.A., Babu T.M., Kottkamp A.C., Luetkemeyer A.F., Immergluck L.C., Presti R.M., Bäcker M., Winokur P.L., Mahgoub S.M., Goepfert P.A., Fusco D.N., Malkin E., Bethony J.M., Walsh E.E., Graciaa D.S., Samaha H., Sherman A.C., Walsh S.R., Abate G., Oikonomopoulou Z., El Sahly H.M., Martin T.C., Kamidani S., Smith M.J., Ladner B.G., Porterfield L., Dunstan M., Wald A., Davis T., Atmar R.L., Mulligan M.J., Lyke K.E., Posavad C.M., Meagher M.A., Stephens D.S., Neuzil K.M., Abebe K., Hill H., Albert J., Telu K., Mu J., Lewis T.C., Giebeig L.A., Eaton A., Netzl A., Wilks S.H., Türeli S., Makhene M., Crandon S., Montefiori D.C., Makowski M., Smith D.J., Nayak S.U., Roberts P.C, & Beigel J.H. (2023). Comparison of bivalent and monovalent SARS-CoV-2 variant vaccines: the phase 2 randomized open-label COVAIL trial. Nature Medicine, 29(9), 2334-2346.

Publication 2023

Elecsys Anti-SARS-CoV-2 N

B 1 1 529 B 1 351 Immunoassays Lentiviruses Mutation Sars cov 2 Sars cov 2 d614g Vaccine

Corresponding Organization : University of Rochester

Other organizations : Emory University, HOPE Clinic, George Washington University, Brigham and Women's Hospital, Harvard University, Saint Louis University, Baylor College of Medicine, University of California, San Diego, Children's Healthcare of Atlanta, Duke University, University of Illinois Chicago, The University of Texas Medical Branch at Galveston, Kaiser Permanente Washington Health Research Institute, Fred Hutch Cancer Center, University of Washington, Manhattan Psychiatric Center, San Francisco General Hospital, University of California, San Francisco, Morehouse School of Medicine, Washington University in St. Louis, Long Island University, University of Iowa, Howard University Hospital, University of Alabama at Birmingham, Tulane University, University of Maryland, Baltimore, Woodruff Health Sciences Center, Family Health International 360, Duke Medical Center, Frederick National Laboratory for Cancer Research, University of Cambridge, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Variable analysis

independent variables

SARS-CoV-2 spike mutations for the D614G (Wuhan-1 containing a single D614G spike mutation), B1.617.2 (Delta), B.1.351 (Beta) and B.1.1.529 (Omicron BA.1) variants
Omicron BA.4/BA.5, BA.2.12.1, BA.2.75.2, BA.2.75, BA.4.6, BF.7 and BQ.1.1 subvariants

dependent variables

SARS-CoV-2 neutralization titers, expressed as the ID50

control variables

Anti-N antibodies detected using Elecsys Anti-SARS-CoV-2 N (Roche) at baseline

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