For each subject, sampling sessions included whole blood collection, passive saliva and exhaled breath condensate collection (41 (link)). Briefly, venous blood was collected in 3mL, ethylenediaminetetraacetic acid-treated (EDTA) tubes (BD Vacutainer) from either the left or right median cubital vein and centrifuged immediately for plasma retrieval. Saliva was collected passively, meaning it was not spit or expectorated but allowed to pool, into sterile 15mL conical centrifuge tubes (Falcon) held against the lips. Rtubes (Respiratory Research), disposable exhaled breath condensers, were used to collect roughly ~2mL of exhaled breath condensate. Biological samples from each matrix were aliquoted into microcentrifuge tubes and stored at −80°C within 20 minutes of collection. Metabolomic analyses were conducted within four months of collection.
Protocol detail
Multimodal Biological Sampling Protocol
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Corresponding Organization : Emory University
Other organizations : Ben-Gurion University of the Negev, Georgia State University
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Variable analysis
independent variables
- Sampling session
- Whole blood collection
- Passive saliva collection
- Exhaled breath condensate collection
- Collection tubes (3mL EDTA-treated tubes for blood, 15mL conical centrifuge tubes for saliva, Rtube disposable exhaled breath condensers)
- Centrifugation and immediate plasma retrieval for blood samples
- Passive saliva collection (not spit or expectorated)
- Storage of biological samples at -80°C within 20 minutes of collection
- Metabolomic analyses conducted within four months of collection
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