Bile Acids Quantification Protocol

Bile acids were analyzed using the commercially available Biocrates Bile Acids Kit (Biocrates Life Science AG, Innsbruck, Austria) as described by Marksteiner et al. [16 (link)]. This assay allows identification and quantification of primary and secondary bile acids and their taurine and glycine conjugated derivatives. The metabolite panel included 17 individual bile acids, corresponding internal standards, and calibration ranges. Data analysis was performed with TargetLynx (Waters) and Biocrates MetIDQ software.

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Yilmaz A., Ugur Z., Ustun I., Akyol S., Bahado-Singh R.O., Maddens M., Aasly J.O, & Graham S.F. (2020). Metabolic Profiling of CSF from People Suffering from Sporadic and LRRK2 Parkinson’s Disease: A Pilot Study. Cells, 9(11), 2394.

Publication 2020

Biocrates Bile Acids Kit TargetLynx

Assay Bile acids Derivatives Glycine Taurine

Corresponding Organization : Oakland University

Other organizations : DePaul University, Norwegian University of Science and Technology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Identification and quantification of primary and secondary bile acids and their taurine and glycine conjugated derivatives

control variables

None explicitly mentioned

controls

Positive controls: Calibration ranges for the 17 individual bile acids and corresponding internal standards
Negative controls: Not explicitly mentioned

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