Reversibility of MAO Inhibitor Kinetics

As previously
stated, the reversibilities of lead compound inhibitions of MAO-A
and MAO-B were assessed after preincubation for 30 min at ∼2
× IC₅₀.^{37 (link)} Reference inhibitors
such as toloxatone (a reversible MAO-A inhibitor), clorgyline (an
irreversible MAO-A inhibitor), lazabemide (a reversible MAO-B inhibitor),
and pargyline (an irreversible MAO-B inhibitor) were also preincubated
at a concentration of ∼2 × IC₅₀ for comparison
with the compounds. Reversibility patterns were identified by comparing
the behaviors of dialyzed (A_D) and undialyzed (A_U) samples.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kumar S., Oh J.M., Abdelgawad M.A., Abourehab M.A., Tengli A.K., Singh A.K., Ahmad I., Patel H., Mathew B, & Kim H. (2023). Development of Isopropyl-Tailed Chalcones as a New Class of Selective MAO-B Inhibitors for the Treatment of Parkinson’s Disorder. ACS Omega, 8(7), 6908-6917.

Publication 2023

Clorgyline Inhibitions Lazabemide Mao b Mao inhibitor Pargyline Toloxatone

Corresponding Organization : Sunchon National University

Other organizations : Al Jouf University, Beni-Suef University, Umm al-Qura University, JSS Academy of Higher Education and Research, ACPM Dental College and Hospital, Shri Vile Parle Kelavani Mandal

Top 5 similar protocols

Variable analysis

independent variables

Preincubation time (30 min)
Preincubation concentration (around 2 × IC50)

dependent variables

Reversibility patterns of lead compound inhibitions of MAO-A and MAO-B

control variables

Preincubation concentration (around 2 × IC50) for reference inhibitors (toloxatone, clorgyline, lazabemide, pargyline)

controls

Positive controls: Reference inhibitors (toloxatone, clorgyline, lazabemide, pargyline)
Negative controls: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!