Vessels were incubated with various inhibitors for 30 minutes prior to conducting PE, ACh or SNP response curves. NO synthase inhibitor, NW-nitro-L-arginine methyl ester (L-NAME 100 μM; Sigma Aldrich), NADPH oxidase was inhibited by a non-specific inhibitor (Apocynin 100 μM) and a specific and novel inhibitor, 3-Benzyl-7-(2-benzoxazolyl)thio-1,2,3-triazolo(4,5-d)pyrimidine (VAS2870 10 μM; both from Sigma Aldrich St. Louis, MO). 1H-[2 (link),12 (link),55 (link)] Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ 10 μM; Sigma Aldrich St. Louis, MO), a guanylyl cyclase inhibitor, was also incubated with some vessels for 30 minutes prior to usage of PE, ACh or SNP. To protect against the potential deleterious effects of ROS, separate vessels, ex vivo, were incubated for 30 minutes with an antioxidant, N-acetyl cysteine (NAC 20 mM; Sigma Aldrich, St. Louis, MO), and the above myograph procedure was conducted as described above.
Protocol detail
Vascular Function Assessment with Inhibitors
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
NW-nitro-L-arginine methyl ester (L-NAME
Apocynin
3-Benzyl-7-(2-benzoxazolyl)thio-1,2,3-triazolo(4,5-d)pyrimidine (VAS2870
Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ
N-acetyl cysteine (NAC
Antioxidant
Apocynin
Arginine methyl ester
Guanylyl cyclase
Inhibitors
L name
Myograph
N acetyl cysteine
Nadph oxidase
No synthase
Pyrimidine
Quinoxalin
Vas2870
Vessels
Corresponding Organization :
Other organizations : New York University, Lanzhou University, Research Triangle Park Foundation, Environmental Protection Agency
Top 5 similar protocols
Variable analysis
independent variables
- Incubation with various inhibitors for 30 minutes prior to conducting PE, ACh or SNP response curves
- NO synthase inhibitor, NW-nitro-L-arginine methyl ester (L-NAME 100 μM)
- NADPH oxidase inhibitor, Apocynin (100 μM) and VAS2870 (10 μM)
- Guanylyl cyclase inhibitor, 1H-[2 (),12 (),55 ()] Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ 10 μM)
- Antioxidant, N-acetyl cysteine (NAC 20 mM)
- PE, ACh or SNP response curves
- Vessel incubation duration (30 minutes)
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!