Biliary ducts or sorted Lgr5-LacZ+ cells were isolated, mixed with Matrigel (BD Bioscience) and cultured as described in ref. 2 (link). Medium composition was AdDMEM/F12 (Invitrogen) supplemented with B27 and N2 (Invitrogen), N-Acetylcysteine (1.25 μM, Sigma), gastrin (10 nM, Sigma), EGF (50 ng/ml, Peprotech), 10% RSPO1 Conditioned Medium (kindly provided by Calvin Kuo), FGF10 (100 ng/ml, Peprotech), nicotinamide (10 mM, Sigma) and HGF (50 ng/ml, Peprotech). Detailed protocols are provided in Supplementary Methods .
Protocol detail
Culturing Lgr5+ Cells in 3D Matrix
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Utrecht University, Oregon Health & Science University, Keio University, Hubrecht Institute for Developmental Biology and Stem Cell Research, Baylor College of Medicine
Top 5 similar protocols
Protocol cited in 42 other protocols
Variable analysis
independent variables
- Biliary ducts or sorted Lgr5-LacZ+ cells
- Growth and differentiation of biliary ducts or sorted Lgr5-LacZ+ cells
- Matrigel (BD Bioscience)
- AdDMEM/F12 (Invitrogen) supplemented with B27 and N2 (Invitrogen), N-Acetylcysteine (1.25 μM, Sigma), gastrin (10 nM, Sigma), EGF (50 ng/ml, Peprotech), 10% RSPO1 Conditioned Medium (kindly provided by Calvin Kuo), FGF10 (100 ng/ml, Peprotech), nicotinamide (10 mM, Sigma) and HGF (50 ng/ml, Peprotech)
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!