APOE Genotyping Protocol for Patient Samples

APOE genotyping was performed on blood samples from all patients and control individuals. Fresh whole blood was drawn into 6 mL EDTA-vacutainers, and DNA isolated using the QIAamp DNA Blood Mini Kit (QIAGEN), together with the spin protocol provided. Random samples of isolated DNA were checked for purity using NanoDrop technology, and all samples were frozen and stored at −80°C. APOE analysis was either performed according to the protocol described elsewhere5 (link) or using the Fast Start DNA Master HybProbe Kit (Roche) in combination with the LightMix ApoE C112R R158C kit from TiB MolBiol (Berlin, Germany) according to the manufacturer's instructions, followed by APOE genotyping with LightCycler technology (Roche).

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Berge G., Sando S.B., Rongve A., Aarsland D, & White L.R. (2014). Apolipoprotein E ε2 genotype delays onset of dementia with Lewy bodies in a Norwegian cohort. Journal of Neurology, Neurosurgery, and Psychiatry, 85(11), 1227-1231.

Publication 2014

QIAamp DNA Blood Mini Kit Fast Start DNA Master HybProbe Kit LightMix ApoE C112R R158C kit LightCycler technology

Apoe Blood Edta Frozen Patients

Corresponding Organization : Norwegian University of Science and Technology

Other organizations : St Olav's University Hospital, Privatsykehuset Haugesund, Akershus University Hospital, Stavanger University Hospital, Karolinska Institutet

Top 5 similar protocols

Variable analysis

independent variables

APOE genotyping

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Blood samples from all patients and control individuals
Fresh whole blood drawn into 6 mL EDTA-vacutainers
DNA isolated using the QIAamp DNA Blood Mini Kit (QIAGEN), together with the spin protocol provided
Random samples of isolated DNA checked for purity using NanoDrop technology
All samples frozen and stored at −80°C

positive controls

Protocol described elsewhere5 (link provided)

negative controls

Not explicitly mentioned

Annotations

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