A cDNA library of Eureka lemon was constructed as previously described (Yang et al., 2021 (link)). Full‐length CYVCV CP (KP313240.1) was amplified with the primers BD‐CP F/R (Table S1) and digested with EcoRI and BamHI. The digested product was cloned into the pGBKT7 vector (Oebiotech) to generate the bait vector BD‐CP. After sequencing verification, proteins interacting with CP were screened from the Eureka lemon library using the Matchmaker Yeast Two‐Hybrid System, as described by Cheng et al. (2008 (link)). Briefly, 5 μL of BD‐CP bait, 5 μL of salmon sperm DNA, and 10 μL of the Eureka lemon cDNA library were used to transform 100 μL of competent yeast cells. The transformants were tested for growth on synthetic dropout (SD−Leu/−Trp) selective medium (SD/−Leu/−Trp and SD/−Leu/−Trp/−His/−Ade supplemented with 200 ng/mL Aureobasidin A and 20 μg/mL X‐α‐Gal) at 30°C (Shalmani et al., 2021 (link)). Three days later, yeast colonies were selected for DNA sequencing and analysed using BLASTn and BLASTx. Full‐length ClRPS9‐2 (XM_006482510.2) was amplified and cloned into the pGADT7 vector (Oebiotech) at the EcoRI and BamHI sites to obtain the prey vector AD‐ClRPS9‐2. The recombinant AD‐ClRPS9‐2 and DB‐CP constructs were cotransformed into the yeast strain Y2H Gold, as described by Wang et al. (2017 (link)).