RT-PCR Analysis of Gene Expression

RT-PCR was performed using SupersciptII (Invitrogen) as described before [50 (link)]. The applied primers were: CCTTACAAGAATGCCGCTCG and TCTCCAGCGGAAAACAGAGC for Ndufs 5, TGTCATCACAACAGGGAGCC and CATGGGGCAGTGTCTTGACT for Nwd1, AGAGCTATGTCGCCCTATGC and GGGGACTTGAGGCATGGATC for Ecm2, GCTGACTGGCCAATGATCCT and AGACACTGCAAACCACTCCC for LOC103694869, TTGTTGTCAAGGACCGGGAG and TCTCTAGACCGCCCATACCC for Rbm3, CTTGGATGGAGCGGACACAT and AGGAGCTGAACTGTTCTGGC for Mepce, AGGGCAATAACCACCAAGCA and GGGCTGTCGTGGCATATTTG for Gpr34 and TGCCACTCAGAAGACTGTGG and GTCCTCAGTGTAGCCCAGGA for GAPDH.

Free full text: Click here

Lékó A.H., Kumari R., Dóra F., Keller D., Udvari E.B., Csikós V., Renner É, & Dobolyi A. (2021). Transcriptome Sequencing in the Preoptic Region of Rat Dams Reveals a Role of Androgen Receptor in the Control of Maternal Behavior. International Journal of Molecular Sciences, 22(4), 1517.

Publication 2021

SupersciptII

Gapdh Primers Rt pcr

Corresponding Organization : Eötvös Loránd University

Other organizations : Semmelweis University

Top 5 similar protocols

Variable analysis

independent variables

Primers for Ndufs 5, Nwd1, Ecm2, LOC103694869, Rbm3, Mepce, Gpr34, and GAPDH

dependent variables

Expression levels of Ndufs 5, Nwd1, Ecm2, LOC103694869, Rbm3, Mepce, Gpr34 measured using RT-PCR

control variables

RT-PCR method using SupersciptII (Invitrogen) as described before [50]

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!