The three different sets of
glass slides, one on which the biofilm was allowed to develop in the
absence of a drug, those which were incubated in the presence of a
drug (to detect inhibition), and those on which biofilm was first
allowed to develop before incubation in the presence drugs (to detect
eradication), were subjected to fluorescence staining, Calcofluor
White Stain (Sigma-Aldrich). The images of biofilm were visualized
under a fluorescence microscope (Nikon Eclipse- excitation filter:
340 ± 380 nm, dichroic mirror: 400 nm, and barrier filter: 435
± 485 nm).56 (link)
glass slides, one on which the biofilm was allowed to develop in the
absence of a drug, those which were incubated in the presence of a
drug (to detect inhibition), and those on which biofilm was first
allowed to develop before incubation in the presence drugs (to detect
eradication), were subjected to fluorescence staining, Calcofluor
White Stain (Sigma-Aldrich). The images of biofilm were visualized
under a fluorescence microscope (Nikon Eclipse- excitation filter:
340 ± 380 nm, dichroic mirror: 400 nm, and barrier filter: 435
± 485 nm).56 (link)
