Kinesin, Microtubule, and Actin Assay
Corresponding Organization :
Other organizations : Technical University of Munich, Center for NanoScience, Ludwig-Maximilians-Universität München, Center for Integrated Protein Science Munich
Variable analysis
- Biotin–kinesin and MTs stabilized with GMP-CPP (Guanosine-5'-[(α,β)-methyleno]triphosphate)
- Not explicitly mentioned
- Kinesin, once thawed, was kept on ice
- MTs were kept at room temperature
- Monomeric actin was stored at 4 °C in the G-Buffer (2 mM Tris, 0.2 mM ATP, 0.2 mM CaCl2, 0.2 mM DTT and 0.005% NaN3 at pH 8.0)
- Gelsolin was purified from adult bovine serum (Sigma-Aldrich)
- No rabbits were directly involved in the study
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