Cultures of Trypanosoma cruzi epimastigotes (RA strain) were maintained through weekly passages in LIT medium supplemented with 10% fetal bovine serum at 28 °C [28 (link)]. Trypanosoma cruzi bloodstream trypomastigotes (RA strain) were obtained from infected CF1 mice by cardiac puncture at the peak of parasitaemia 15 days after infection and transfected trypomastigotes expressing β-galactosidase (Clone C4, e Tulahuen strain) were obtained from infected Vero cells [7 (link)].
Corresponding Organization : Consejo Nacional de Investigaciones Científicas y Técnicas
Other organizations :
Fundación Ciencias Exactas y Naturales, Instituto de Histología y Embriología de Mendoza, Universidad Nacional de Cuyo, National University of Tucumán, National University of General San Martín, Universidad de la República
Transfected trypomastigotes expressing β-galactosidase (Clone C4, e Tulahuen strain)
dependent variables
Not explicitly mentioned
control variables
LIT medium supplemented with 10% fetal bovine serum
Temperature maintained at 28 °C
Infected CF1 mice
Infected Vero cells
controls
Positive control: Infected Vero cells expressing β-galactosidase (Clone C4, e Tulahuen strain)
Negative control: Not explicitly mentioned
Annotations
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