BC cell lines BT-474 and BT-20 (cat. nos. 300130 and 300131; CLS Cell Lines Service GmbH), HS-578T (cat. no. 86082104; Sigma-Aldrich; Merck KGaA) and MDA-MB-231 (cat. no. 92020424; Sigma-Aldrich; Merck KGaA) (46 (link)) were incubated in DMEM/F12 medium (Gibco; Thermo Fisher Scientific, Inc.), supplemented with 5% FBS (Gibco; Thermo Fisher Scientific, Inc.), 1% HEPES buffer (Gibco; Thermo Fisher Scientific, Inc.) and 1% 100 U/ml penicillin/streptomycin (Sigma-Aldrich; Merck KGaA). Insulin (2.5%; Insuman® rapid; Sanofi S.A.) was added to BT-474 cells. Cells were incubated under humidified conditions at 37°C and 5% CO2. The cell lines were authenticated using PCR-single-locus-technology. Mycoplasma testing is performed regularly.
Cells were treated with 2.0 μg/ml carboplatin (Abmole Biosciences Inc.), 1.0 μg/ml epirubicin (Sigma-Aldrich; Merck KGaA), 40.0 μg/ml gemcitabine (Abmole Bioscience Inc.) or 2.0 μg/ml paclitaxel (Sigma-Aldrich; Merck KGaA) in DMSO-free solution for 18 h. Untreated cells served as a control.
Cells were treated with 2.0 μg/ml carboplatin (Abmole Biosciences Inc.), 1.0 μg/ml epirubicin (Sigma-Aldrich; Merck KGaA), 40.0 μg/ml gemcitabine (Abmole Bioscience Inc.) or 2.0 μg/ml paclitaxel (Sigma-Aldrich; Merck KGaA) in DMSO-free solution for 18 h. Untreated cells served as a control.
