Cell Lysate Protein Analysis

After the desired cell treatment extraction of total cell lysates, SDS-polyacrylamide electrophoresis and immunoblot analysis were performed essentially as described previously (24 (link)). Enhanced chemoluminescence-based image acquisition was done on a Fuji LAS300 system coupled with densitometric analysis in the Multi Gauge software.

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Norden E, & Heiss E.H. (2018). Urolithin A gains in antiproliferative capacity by reducing the glycolytic potential via the p53/TIGAR axis in colon cancer cells. Carcinogenesis, 40(1), 93-101.

Publication 2019

LAS300 system

After treatment Cell Densitometric Electrophoresis Immunoblot analysis Polyacrylamide

Corresponding Organization : University of Vienna

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Variable analysis

independent variables

Cell treatment

dependent variables

Protein expression/abundance (measured by immunoblot analysis and densitometric analysis)

control variables

Experimental procedures (SDS-polyacrylamide electrophoresis and immunoblot analysis) were performed as described previously (24)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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