Blood stage parasite infections were conducted in female Swiss mice (6–8 weeks old, from Janvier). Sporozoite infections were performed in female C57BL/6 mice (6 weeks old, from Janvier). We used the reference rodent malaria parasite strains P. berghei ANKA (clone 15cy1) and P. yoelii 17XNL (clone 1.1). Anopheles stephensi mosquitoes were infected by feeding on anesthetised infected mice using standard methods of mosquito infection27 (link). P. berghei- and P. yoelii-infected mosquitoes were kept at 21°C and 24°C, respectively, and fed daily on 10% sucrose. After 21 to 28 days (for P. berghei) or 14 to 18 days (for P. yoelii), the salivary glands of the mosquitoes were collected by hand-dissection and homogenized to collect sporozoites. HepG2 cells (ATCC HB-8065) were cultured in DMEM supplemented with 10% fetal calf serum and antibiotics, as described28 (link).
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Rodent Malaria Parasite Infection Protocols
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Anopheles
Antibiotics
Blood
C57bl mice
Clone
Dissection
Female
Fetal calf serum
Hepg2 cells
Infections
Malaria
Mice
Mosquitoes
Parasite
Parasite infections
Rodent
Salivary glands
Sporozoites
Strains
Sucrose
Swiss mice
Corresponding Organization :
Other organizations : Centre d'Immunologie et des Maladies Infectieuses, Sorbonne Université, Inserm
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Variable analysis
independent variables
- Mouse strain (female Swiss mice, female C57BL/6 mice)
- Malaria parasite strain (P. berghei ANKA, P. yoelii 17XNL)
- Parasite infection
- Sporozoite infection
- Sporozoite collection
- Age of mice (6-8 weeks for Swiss mice, 6 weeks for C57BL/6 mice)
- Source of mice (Janvier)
- Mosquito infection method (anesthetized infected mice)
- Mosquito temperature (21°C for P. berghei, 24°C for P. yoelii)
- Mosquito feeding (10% sucrose)
- Time for sporozoite collection (21-28 days for P. berghei, 14-18 days for P. yoelii)
- HepG2 cell culture conditions (DMEM with 10% fetal calf serum and antibiotics)
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