Glucose uptake was measured using a kit from Cayman Chemicals (600470) and used as previously described11 (link). For all lysates, 100 μL were loaded in triplicate onto a 96-well plate and a fluorescence reading of 485/535 was taken (Biotek Synergy Neo model NEOALHPA B, Gen 5 software). The concentration of glucose was determined using a standard curve and results are reported as micrograms per milliliter per 105 cells.
Protocol detail
Quantifying Cellular Glucose Uptake
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Corresponding Organization :
Other organizations : University of Iowa, Institut Jacques Monod, Université Paris Cité, Centre National de la Recherche Scientifique
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Variable analysis
independent variables
- None explicitly mentioned
- Glucose uptake
- Cell number (10^5 cells)
- None explicitly mentioned
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