Glucose uptake was measured using a kit from Cayman Chemicals (600470) and used as previously described11 (link). For all lysates, 100 μL were loaded in triplicate onto a 96-well plate and a fluorescence reading of 485/535 was taken (Biotek Synergy Neo model NEOALHPA B, Gen 5 software). The concentration of glucose was determined using a standard curve and results are reported as micrograms per milliliter per 105 cells.