Mass Cytometry Sample Preparation

Intercalated samples were washed with once with CSM and twice with
double distilled water, and then re-suspended in a solution of normalization
beads (Fluidigm). Samples were filtered prior to mass cytometry analysis through
a 35-µm membrane and analyzed at a flow rate of approximately 500
cells/sec. Samples were normalized and de-barcoded as described previously
(15 (link),16 (link)).

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Fragiadakis G.K., Baca Q.J., Gherardini P.F., Ganio E.A., Gaudilliere D.K., Tingle M., Lancero H.L., McNeil L.S., Spitzer M.H., Wong R.J., Shaw G.M., Darmstadt G.L., Sylvester K.G., Winn V.D., Carvalho B., Lewis D.B., Stevenson D.K., Nolan G.P., Aghaeepour N., Angst M.S, & Gaudilliere B.L. (2016). Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy. Journal of immunology (Baltimore, Md. : 1950), 197(11), 4482-4492.

Publication 2016

normalizationbeads

Membrane

Corresponding Organization : Stanford University

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Washing of intercalated samples with CSM and double distilled water
Re-suspension of samples in a solution of normalization beads (Fluidigm)
Filtering of samples prior to mass cytometry analysis through a 35-µm membrane
Analysis at a flow rate of approximately 500 cells/sec

dependent variables

Mass cytometry analysis

control variables

Normalization and de-barcoding of samples as described previously (15, 16)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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