The Y82E9BR.3-specific RNAi clone, which was prepared previously21 (link), was cultured overnight in LB broth containing 50 μg/ml ampicillin (USB, Santa Clara, CA, USA). To induce double-stranded RNA, 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology, St. Louis, MO, USA) was added to plates for incubation at room temperature for 1 day before usage.
Free full text: Click here