The effect of curcumin on cell proliferation was determined by using TACS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay (Trevigen, Gaithersburg) as previously described [15 (link)]. Wound-healing assay and in vitro apoptosis on MDA.MB231 cells were performed as previously described [15 (link), 33 (link)].
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