The amphiphilic copolymer (PMAO-grafted-PEG)
was previously synthesized by binding long poly(ethylene glycol)-amine
(PEG-NH2) tales (5–20 kDa) in a high density into
the poly(maleic anhydride-alt-1-octadecene) PMAO
backbone, specifically 75% of PMAO monomers are used in the grafting
reaction. The standard procedure used to coat the FM-NPs with PMAO
or PMAO-g-PEG was carried out based on the procedure
described elsewhere.48 (link) In order to get
the FM-NPs individually or quasi-individually coated, the procedure
was modified by a systematic study of several parameters. The protocol
was refined through the analysis of the dynamical hysteresis loops
and SAR values of different preparations in which one parameter was
changed at a time (Table S3 and Figure S1 in Supporting Information). Briefly,
in an optimized protocol the stock solution of FM-NPs in CHCl3 (c ∼ 20 mg/mL) is added dropwise
to the PMAO–PEG solution in CHCl3 (c ∼ 5 mgPMAO/ml) using 50 monomers of PMAO per nm2 of NP surface. The mixture is stirred for 30 min, and the
solvent is evaporated in a rotavapor. Finally, the hydrolysis of the
remaining maleic anhydride groups in the polymeric surface is completed
by adding sodium borate buffer at pH = 9. The Z potential and the
hydrodynamic radius of the samples presented in the article are summarized
inTable S2 , Supporting Information.
was previously synthesized by binding long poly(ethylene glycol)-amine
(PEG-NH2) tales (5–20 kDa) in a high density into
the poly(maleic anhydride-alt-1-octadecene) PMAO
backbone, specifically 75% of PMAO monomers are used in the grafting
reaction. The standard procedure used to coat the FM-NPs with PMAO
or PMAO-g-PEG was carried out based on the procedure
described elsewhere.48 (link) In order to get
the FM-NPs individually or quasi-individually coated, the procedure
was modified by a systematic study of several parameters. The protocol
was refined through the analysis of the dynamical hysteresis loops
and SAR values of different preparations in which one parameter was
changed at a time (
in an optimized protocol the stock solution of FM-NPs in CHCl3 (c ∼ 20 mg/mL) is added dropwise
to the PMAO–PEG solution in CHCl3 (c ∼ 5 mgPMAO/ml) using 50 monomers of PMAO per nm2 of NP surface. The mixture is stirred for 30 min, and the
solvent is evaporated in a rotavapor. Finally, the hydrolysis of the
remaining maleic anhydride groups in the polymeric surface is completed
by adding sodium borate buffer at pH = 9. The Z potential and the
hydrodynamic radius of the samples presented in the article are summarized
in
