Cold maceration technique was used for the extraction of plant material and a total of 200 g of the coarse powder was used. During the process, 100 g of the coarse powder was soaked in an Erlenmeyer flask with 1 L of distilled water and then placed on a shaker (Bibby Scientific Limited Stone Staffo Reshire, UK) tuned to 120 rpm with occasional shaking for 72 h at room temperature. The extract was filtered first using a muslin cloth and then Whatman grade No-1 filter paper and the marc was re-macerated for a second and third time by adding another fresh solvent [27 (link)]. The filtrates were left overnight in a deep freezer and then lyophilized using freeze dryer. After drying, percentage yield of crude aqueous stem extract of L. camara was determined to be 16.7% w/w. The dried plant extract was reconstituted with distilled water for oral administration.
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