Assessing Anxiety-like Behavior in Mice

The open field test (OFT) was used to assess anxiety-like behaviors. The open field area in this experiment was an enclosed area surrounded by black plexiglass (60 × 60 × 30 cm). Each mouse was gently placed in the same corner of the arena facing the same direction and allowed to freely explore the arena for 10 min. The time spent in the center square and track movements of mice were recorded by a video camera system (SMART 3.0; Panlab S. L., Barcelona, Spain). The criterion for entering the center square was defined as 50% of the body being positioned within this square.

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Hou L., Yang L., Zhu C., Miao J., Zhou W., Tang Y., Meng H, & Liu S. (2023). Cuscutae semen alleviates CUS-induced depression-like behaviors in mice via the gut microbiota-neuroinflammation axis. Frontiers in Pharmacology, 14, 1107781.

Publication 2023

Anxiety behaviors Body Mice Movements Open field test Plexiglass

Corresponding Organization :

Other organizations : Shandong University

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Variable analysis

independent variables

Position of the mouse in the arena (same corner, facing same direction)

dependent variables

Time spent in the center square
Track movements of mice

control variables

Size of the open field arena (60 × 60 × 30 cm)
Enclosure material (black plexiglass)
Duration of the test (10 minutes)

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

Based on most similar protocols

The open field area should be an enclosed area surrounded by dark opaque plexiglass to minimize external cues that may affect anxiety-like behaviors (Input, Protocol 1, Protocol 3).

The size of the open field arena should be consistent across protocols, typically between 40 cm x 40 cm to 60 cm x 60 cm (Input, Protocol 1, Protocol 2, Protocol 3, Protocol 4, Protocol 5).

The height of the walls surrounding the open field arena should be consistent, typically between 30 cm to 40 cm, to prevent the mice from escaping (Input, Protocol 1, Protocol 2, Protocol 3, Protocol 4).

The mice should be gently placed in the same corner of the arena facing the same direction to ensure consistency in the starting position (Input, Protocol 1, Protocol 2).

The time spent in the center square and track movements of the mice should be recorded using a video camera system (Input, Protocol 1, Protocol 2, Protocol 3, Protocol 4, Protocol 5).

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As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

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