Mosquito Genotyping via Microsatellite Analysis

Total DNA was extracted using the DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer instructions, with an additional RNAse A (Qiagen) step. DNA samples were stored at -20^°C until further analysis. Individual mosquitoes were genotyped as described in Brown et al. [13 (link)] for twelve previously published [2 (link),13 (link),14 ] microsatellite loci [A1, B2, B3, A9 (tri-nucleotide repeats), and AC2, CT2, AG2, AC4, AC1, AC5, AG1, and AG4 (di-nucleotide repeats)]. Microsatellite alleles were scored using GENEMAPPER version 4.0 (Applied Biosystems). Raw allele frequencies are available at VectorBase.org, Project ID VBP0000176 and are also available as Supporting Information (S1 Appendix).

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Kotsakiozi P., Gloria-Soria A., Caccone A., Evans B., Schama R., Martins A.J, & Powell J.R. (2017). Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses. PLoS Neglected Tropical Diseases, 11(7), e0005653.

Publication 2017

DNeasy Blood and Tissue kit RNAse A GENEMAPPER version 4.0

Alleles Blood Microsatellite Mosquitoes Nucleotide Rnase Tissue

Corresponding Organization : Fundação Oswaldo Cruz

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Variable analysis

independent variables

Microsatellite loci (A1, B2, B3, A9 (tri-nucleotide repeats), and AC2, CT2, AG2, AC4, AC1, AC5, AG1, and AG4 (di-nucleotide repeats))

dependent variables

Microsatellite allele frequencies

control variables

Total DNA extraction using the DNeasy Blood and Tissue kit (Qiagen) with an additional RNAse A step
DNA samples stored at -20°C until further analysis

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