MRC5 and Efk3 cells were seeded at a concentration of 3 × 105 cells/well in six-well plates and transfected with 750 ng/mL poly(I:C) (InvivoGen, San Diego, CA, USA) using Lipofectamine 2000 (Invitrogen, Camarillo, CA, USA) as previously described [38 (link)]. Briefly, 750 ng/mL poly(I:C) was mixed in a total volume of 250 μL of TransfectaGro (Corning) and 12 μL of Lipofectamine 2000. This mixture was incubated at room temperature for 15 min and added to cells in complete medium. Cells were harvested 16 h post-transfection and RNA was extracted.
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