The analysis of aminoglycosides in the sewage samples was performed as in Bello53 (link), with adaptations as summarized in the following. For sample pretreatment, two 10-mL portions of each sample of sewage were weighed into separate 50-mL tubes, after which internal standards were added. To one of these portions, aminoglycosides were added at a level of 50 μg/L. Twenty mL of extraction liquid (10 mM KH2PO4 with 0.4 mM EDTA and 2% TCA) were added, and samples were mixed by means of a vortex and shaken head-over-head for 10 min. The extract was then brought to pH 7.6–7.9 and centrifuged (15 min, 3600 × g). The complete extract was transferred to a conditioned CBX cartridge, followed by washing with 4 mL of water and drying. The aminoglycosides were eluted with 3 mL of acetic acid (10% in MeOH). The eluate was dried at 60 °C, evaporated under N2 and taken up in 400 μL of HFBA (0.065%). For LC-MS/MS, the following gradient was applied: 0–0.5 min, 0% B; 0.5–5 min, linear increase to 45% B; 5–8 min, linear increase to 60% B; 8–10 min, linear increase to 100% B. The injection volume was 40 μL. Detection was carried out by a Waters (Milford, MA, USA) Quattro Ultima mass spectrometer in positive ESI mode. The parameters used were: capillary voltage, 2.7 kV; desolvation temperature, 500 °C; source temperature, 120 °C; cone gas, 150 L/h; and desolvation gas 550 L/h.
Other organizations :
Technical University of Denmark, University of Edinburgh, Erasmus University Rotterdam, Wageningen University & Research, National Institute for Public Health and the Environment, Melbourne Water, Canberra Hospital, Austrian Agency for Health and Food Safety, Botswana International University of Science and Technology, National Center of Infectious and Parasitic Diseases, Institut Pasteur du Cambodge, University of Regina, Guangdong Provincial Center for Disease Control and Prevention, Shantou University, Shantou University Medical College, Colombian Corporation for Agricultural Research - AGROSAVIA, Institut Pasteur de Côte d'Ivoire, Andrija Stampar Teaching Institute of Public Health, University of Zagreb, Instituto Nacional de Investigación en Salud Pública, Addis Ababa University, University of Helsinki, National Center for Disease Control and Public Health, Robert Koch Institute, University for Development Studies, Semmelweis University, University of Veterinary Medicine, University of Iceland, Cochin University of Science and Technology, Shahid Beheshti University of Medical Sciences, Ollscoil na Gaillimhe – University of Galway, Ben-Gurion University of the Negev, Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Mount Kenya University, Kenya Medical Research Institute, University of Prishtina, Institute of Food Safety, Animal Health and Environment “BIOR”, Luxembourg Institute of Science and Technology, Ministry for Health, AIMST University, Agriculture and Forestry University, University of Otago, University of Ibadan, Norwegian Veterinary Centre for Contract Research and Commercial Services, Aga Khan University, National Institute of Quality, National Veterinary Research Institute, Institute of public health of Republic of Macedonia, Pharmaceutical Formulations (United States), Institut Pasteur de Dakar, Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Public Health Authority of the Slovak Republic, National Laboratory of Health, Environment and Food, University of the Witwatersrand, Centre de Recerca en Sanitat Animal, Swedish University of Agricultural Sciences, University of Gothenburg, Kilimanjaro Christian Medical Centre, Ministry of Health and Social Welfare, Togolese Ministry of Health, University of Washington, University of Zambia, University of N'Djamena, Norwegian Institute of Public Health, Catholic University of the Maule, European Bioinformatics Institute, World Health Organization
Positive control: The sample portion with added aminoglycosides at 50 μg/L
Negative control: The sample portion without added aminoglycosides
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
