A total of 77 K-serotype Klebsiella reference strains purchased from Statens Serum Institute, Copenhagen, Denmark. An additional strain (A1517) of novel type KN1 was identified in a previous study from our laboratory [19] (link). Another eleven K. pneumoniae clinical isolates were obtained from Taiwanese and overseas clinical laboratories, including National Taiwan University Hospital (NTUH; Taipei, Taiwan), En Chu Kong Hospital (ECKH; Sansia, Taiwan), Far Eastern Memorial Hospital (FEMH; Banciao, Taiwan), Chang Gung Memorial Hospital (CGMH; Linkou, Taiwan), Department of Medical Microbiology, University of Manitoba (Winnipeg, MB, Canada), and Department of Clinical Microbiology, Kuopio University Hospital (Finland) [19] (link). Together, strains representing the 78 known capsular types were included for wzc sequencing.
Between 2004 and 2006, Twenty-nine strains were collected from the blood of patients admitted to NTUH with bacteremia. To evaluate the wzc typing system in typing strains with unknown capsular types, all of the 29 K. pneumoniae clinical isolates of unknown capsular type were screened by wzc sequencing.
A K. pneumoniae clinical isolate from NTUH, NTUH-K2044 (K1), and its isogenic mutants NTUH-K2044 ΔmagA (capsule deficient) and NTUH-K2044 ΔwbbO (O-antigen deficient) [32] (link) were used as controls for Alcian blue staining.
Between 2004 and 2006, Twenty-nine strains were collected from the blood of patients admitted to NTUH with bacteremia. To evaluate the wzc typing system in typing strains with unknown capsular types, all of the 29 K. pneumoniae clinical isolates of unknown capsular type were screened by wzc sequencing.
A K. pneumoniae clinical isolate from NTUH, NTUH-K2044 (K1), and its isogenic mutants NTUH-K2044 ΔmagA (capsule deficient) and NTUH-K2044 ΔwbbO (O-antigen deficient) [32] (link) were used as controls for Alcian blue staining.
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