Comprehensive Western Blot Analysis

Western blotting was performed as previously described (Seo et al., 2017 (link)). Affinity-purified rabbit polyclonal anti-CPNE7 and anti-DSP antibodies were produced as previously described (Lee et al., 2010 (link); Lee et al., 2011 (link)). Anti-LC3 B polyclonal antibody (Abcam, ab48394), anti-TAU monoclonal antibody (TAU-5; Thermo Fisher Scientific, AHB0042), anti-phospho-mTOR (mechanistic target of rapamycin kinase) polyclonal antibody (Cell Signaling Technology, 5536), anti-DMP-1 (dentin matrix protein 1) polyclonal antibody (Abcam, ab103203), anti-NESTIN monoclonal antibody (10C2; Thermo Fisher Scientific, MA1-110), and anti-GAPDH monoclonal antibody (GA1R; Thermo Fisher Scientific, MA5-15738) were purchased from the respective companies. Protein loading was assessed by the expression of GAPDH. All reactions were performed in triplicate. Semi-quantitative analysis were performed using ImageJ software (National Institute of Health).

Free full text: Click here

Park Y.H., Son C., Seo Y.M., Lee Y.S., Har A, & Park J.C. (2021). CPNE7-Induced Autophagy Restores the Physiological Function of Mature Odontoblasts. Frontiers in Cell and Developmental Biology, 9, 655498.

Publication 2021

ab48394 ab103203 anti-GAPDH monoclonal antibody (GA1R;

Anti antibodies Anti antibody Antibody Dentin matrix protein 1 Gapdh Kinase Mechanistic target of rapamycin Monoclonal antibody Nestin Protein Rabbit

Corresponding Organization : Seoul National University

Top 5 similar protocols

Variable analysis

independent variables

Western blotting protocol described in Seo et al., 2017
Affinity-purified rabbit polyclonal anti-CPNE7 and anti-DSP antibodies produced as described in Lee et al., 2010 and Lee et al., 2011

dependent variables

Expression of LC3 B, TAU, phospho-mTOR, DMP-1, and NESTIN proteins

control variables

Protein loading assessed by the expression of GAPDH

controls

Positive control: None specified
Negative control: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!