Molecular Characterization of CiLV-C Isolates

The complete sequence of p29 (795 nts) and partial sequence of p32 (288 nts) in the RNA1 and RNA2, respectively, of CiLV-C isolates, were obtained using described primers (Locali et al., 2003 (link); Ramos-González et al., 2016 (link); Table 2). Amplicons were obtained from 26 samples collected in non-commercial citrus regions in Brazil and Argentina, from 2006 to 2019, and 31 from commercial citrus orchards inside the citrus belt SP-MG, in the period 2017 to 2019 (Supplementary Table 1). After RT-PCR, amplicons were purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, United States), and cloned into pGEM-T-Easy (Promega, Madison, WI, United States). Plasmids were transformed into Escherichia coli DH10β competent cells by electroporation, and 5–10 recombinant clones derived from each sample were sequenced by the Sanger method (Instituto Biológico, SP, Brazil). PCR products from some samples collected in non-commercial orchards (Supplementary Table 1) were directly sequenced after the purification using the Wizard SV Gel and PCR Clean-Up System (Promega).