Proteasome Activity Measurement in Fibroblasts
Corresponding Organization :
Other organizations : National and Kapodistrian University of Athens
Protocol cited in 1 other protocol
Variable analysis
- Proteasome peptidase activities
- Trypsin-like activity (T-L)
- Caspase-like activity (C-L)
- Chymotrypsin-like activity (CT-L)
- Human skin fibroblasts
- VersaFluorTM Fluorometer System (Bio-Rad laboratories, Hercules, CA, USA)
- Boc-Leu-Arg-Arg-AMC-LRR (trypsin-like activity, T-L), Z-Leu-Leu-Glu-AMC-LLE (caspase-like activity, C-L) and Suc-Leu-Leu-Val-Tyr-AMC-LLVY (chymotrypsin-like activity, CT-L) (Enzo Life Sciences, Inc. Farmingdale, NY, USA) fluoropeptides
- No positive or negative controls were explicitly mentioned in the information provided.
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