Proteasome peptidase activities in human skin fibroblasts were measured at a VersaFluorTM Fluorometer System (Bio-Rad laboratories, Hercules, CA, USA) as described previously64 (link) by using the Boc-Leu-Arg-Arg-AMC-LRR (trypsin-like activity, T-L), Z-Leu-Leu-Glu-AMC-LLE (caspase-like activity, C-L) and Suc-Leu-Leu-Val-Tyr-AMC-LLVY (chymotrypsin-like activity, CT-L) (Enzo Life Sciences, Inc. Farmingdale, NY, USA) fluoropeptides.
Free full text: Click here