Maternal-Fetal Serum Sampling Procedure

Blood from healthy pregnant women was collected from the maternal vein before delivery (n = 40) and from the umbilical cord vein immediately after delivery of the placenta (n = 40). Blood samples were collected in S-Monovette serum tubes (Sarstedt AG, Germany) and processed within 1 h. Samples were allowed to clot at RT and then serum was obtained after centrifugation at 2000 g for 10 mins at 4 °C. Serum samples were aliquoted and stored at −20 °C until further use as described in^{77 (link),78 (link)}.

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Melhem H., Kallol S., Huang X., Lüthi M., Ontsouka C.E., Keogh A., Stroka D., Thormann W., Schneider H, & Albrecht C. (2019). Placental secretion of apolipoprotein A1 and E: the anti-atherogenic impact of the placenta. Scientific Reports, 9, 6225.

Publication 2019

S-Monovette serum tubes

Blood Centrifugation Clot Cord Delivery Placenta Pregnant women Serum Umbilical cord Umbilical vein Vein

Corresponding Organization :

Other organizations : University of Bern, University Hospital of Bern

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Variable analysis

independent variables

Blood collection from maternal vein before delivery
Blood collection from umbilical cord vein immediately after delivery of the placenta

dependent variables

Serum samples

control variables

Blood samples collected in S-Monovette serum tubes (Sarstedt AG, Germany)
Samples processed within 1 h
Samples allowed to clot at RT
Serum obtained after centrifugation at 2000 g for 10 mins at 4 °C
Serum samples aliquoted and stored at −20 °C until further use

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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