Antibody Detection of Epitope-Tagged Proteins

The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (BioLegend; 901515) and rabbit polyclonal antibody (pAb) anti-HA (Invitrogen; PI715500). The Ty1 epitope was detected with mouse mAb BB2 [61 (link)]. The c-Myc epitope was detected with mouse mAb 9E10 [62 (link)]. The V5 epitope was detected with mouse mAb anti-V5 (Invitrogen; R96025) and rabbit mAb anti-V5 (Cell Signaling Technology; 13202S). The OLLAS epitope was detected with rat mAb anti-OLLAS [63 (link)]. Toxoplasma-specific antibodies include rabbit pAb anti-IMC6 [64 (link)], mouse mAb anti-IMC1 [65 (link)], mouse mAb anti-ISP1 [32 (link)], rabbit pAb anti-Centrin1 (Kerafast; EBC004) [66 (link)], and rabbit anti-Catalase [67 (link)].

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Pasquarelli R.R., Back P.S., Sha J., Wohlschlegel J.A, & Bradley P.J. (2023). Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii. PLOS Pathogens, 19(10), e1011707.

Publication 2023

HA.11 anti-HA anti-V5

Anti antibody Antibodies Antibody C myc Catalase Epitope Monoclonal antibody Mouse Rabbit Toxoplasma

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

Mouse monoclonal antibody (mAb) HA.11 (BioLegend; 901515)
Rabbit polyclonal antibody (pAb) anti-HA (Invitrogen; PI715500)
Mouse mAb BB2
Mouse mAb 9E10
Mouse mAb anti-V5 (Invitrogen; R96025)
Rabbit mAb anti-V5 (Cell Signaling Technology; 13202S)
Rat mAb anti-OLLAS
Rabbit pAb anti-IMC6
Mouse mAb anti-IMC1
Mouse mAb anti-ISP1
Rabbit pAb anti-Centrin1 (Kerafast; EBC004)
Rabbit anti-Catalase

dependent variables

Detection of HA epitope
Detection of Ty1 epitope
Detection of c-Myc epitope
Detection of V5 epitope
Detection of OLLAS epitope
Detection of Toxoplasma-specific antibodies

control variables

Toxoplasma-specific antibodies

Annotations

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