Quantitative Western Blot Analysis

Protein concentrations were determined using bicinchoninic acid (BCA) assay kit (Thermo Scientific, MA, USA) with bovine serum albumin standard. Samples (30 μg protein per lane) were separated in SDS-polyacrylamide gels and transferred electrophoretically to Immobilon-PSQ transfer membranes (Millipore), as reported previously57 (link). Band intensities were measured using FluorChem^TM SP software (Alpha. Innotech, San Leandro, CA, USA) and normalized to β-actin or total Akt.

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Park H.R., Lee H., Park H., Cho W.K, & Ma J.Y. (2016). Fermented Sipjeondaebo-tang Alleviates Memory Deficits and Loss of Hippocampal Neurogenesis in Scopolamine-induced Amnesia in Mice. Scientific Reports, 6, 22405.

Publication 2016

BCA) assay kit Immobilon-PSQ transfer membranes

Actin Assay Bicinchoninic acid Bovine serum albumin Immobilon Membranes Polyacrylamide gels Protein

Corresponding Organization :

Other organizations : Korea Institute of Oriental Medicine

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Variable analysis

independent variables

Protein concentrations determined using bicinchoninic acid (BCA) assay kit

dependent variables

Band intensities measured using FluorChem™ SP software and normalized to β-actin or total Akt

control variables

Samples (30 μg protein per lane) separated in SDS-polyacrylamide gels and transferred electrophoretically to Immobilon-PSQ transfer membranes

controls

Bovine serum albumin standard used for protein concentration determination

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